Basic Cell Culture Protocols 3rd Edition

Basic Cell Culture Protocols 3rd Edition


Hardcover: 371 pages
Publisher: Humana Press; 3rd Bk&Cdr edition (October 31, 2004)
Language: English
ISBN-10: 1588292843
ISBN-13: 978-1588292841

Table of Contents

1 Culture of Primary Adherent Cells and a Continuously Growing Non-Adherent Cell Line
2 Detection of Mycoplasma Contaminations
3 Eradication of Mycoplasma Contaminations
4 Authentication of Scientific Human Cell Lines: Easy to Use DNA Fingerprinting
5 Cytogenetic Analysis of Cell Lines
6 Human and Mouse Hematopoietic Colony Forming Cell Assays
7 Isolation and Culture of Murine Macrophages
8 Isolation and Culture of Human Macrophages
9 The Development of T Lymphocytes in Mouse Fetal Thymus Organ Culture
10 In Vitro Generation of Lymphocytes from Embryonic Stem Cells
11 Hematopoietic Development of Human Embryonic Stem Cells in Culture
12 Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros (AGM) region
13 Culture of Human and Mouse Mesenchymal Cells
14 Isolation, Purification, and Cultivation of Rat and Human Keratinocytes
15 Isolation and Culture of Primary Human Hepatocytes
16 Primary Kidney Proximal Tubule Cells
17 Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity
18 Generation and Differentiation of Neurospheres from Murine Embryonic Day 14 Central Nervous System Tissue
19 Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells
20 Adult Ventricular Cardiomyocytes: Isolation and Culture
21 Isolation and Culture of Primary Endothelial Cells
22 Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions
23 Isolation and Characterization of SP Cells
24 Scalable Production of Embryonic Stem Cell-Derived Cells


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