Laser Capture Microdissection: Methods and Protocols

Laser Capture Microdissection: Methods and Protocols



Publisher: Humana Press
Number Of Pages: 310
Publication Date: 2004-12-23
Sales Rank: 995919
ISBN / ASIN: 1588292606
EAN: 9781588292605
Binding: Hardcover
Book Description:

Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.


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